Rational Design of a Highly Selective Near-Infrared Two-Photon Fluorogenic Probe for Imaging Orthotopic Hepatocellular Carcinoma Chemotherapy
- 주제(키워드) activatable probes , analytical methods , fluorogenic imaging , orthotopic hepatocellular carcinoma , specific substrates
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Wu, Xiaofeng; Qi, Sujie; Kwon, Nahyun; Han, Jingjing; Kim, Heejeong; Li, Haidong; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03706, South Korea; [Wang, Rui; Yu, Fabiao] Hainan Med Univ, Affiliated Hosp 1, Coll Emergency & Trauma,Key Lab Hain Trauma & Dis, Inst Funct Mat & Mol Imaging,Minst Educ,Key Lab E, Haikou 571199, Peoples R China
- 등재 SCIE, SCOPUS
- 발행기관 WILEY-V C H VERLAG GMBH
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000181628
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/anie.202101190
초록/요약
Selective fluorescence imaging of biomarkers in vivo and in situ for evaluating orthotopic hepatocellular carcinoma (HCC) chemotherapy remains a great challenge due to current imaging agents suffering from the potential interferences of other hydrolases. Herein, we observed that carbamate unit showed a high selectivity toward the HCC-related biomarker carboxylesterase (CE) for evaluation of treatment. A near-infrared two-photon fluorescent probe was developed to not only specially image CE activity in vivo and in situ but also target orthotopic liver tumor after systemic administration. The in vivo signals of the probe correlate well with tumor apoptosis, making it possible to evaluate the status of treatment. The probe enables the imaging of CE activity in situ with a high-resolution three-dimensional view for the first time. This study may promote advances in optical imaging approaches for precise imaging-guided diagnosis of HCC in situ and its evaluation of treatment.
more