Enhancing acid tolerance of Escherichia coli via viroporin-mediated export of protons and its application for efficient whole-cell biotransformation
- 주제(키워드) (Z)-11-(heptanolyoxy)undec-9-enoic acid , 2′-Fucosyllactose , Acid tolerance , Influenza A matrix-2 protein , Whole-cell biotransformation
- 등재 SCIE, SCOPUS
- 발행기관 Academic Press Inc.
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182148
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.ymben.2021.07.007
- PubMed https://pubmed.ncbi.nlm.nih.gov/34280569
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Escherichia coli-based whole-cell biocatalysts are widely used for the sustainable production of value-added chemicals. However, weak acids present as substrates and/or products obstruct the growth and fermentation capability of E. coli. Here, we show that a viroporin consisting of the influenza A matrix-2 (M2) protein, is activated by low pH and has proton channel activity in E. coli. The heterologous expression of the M2 protein in E. coli resulted in a significant increase in the intracellular pH and cell viability in the presence of various weak acids with different lengths of carbon chains. In addition, the feasibility of developing a robust and efficient E. coli-based whole-cell biocatalyst via introduction of the proton-selective viroporin was explored by employing (Z)-11-(heptanolyoxy)undec-9-enoic acid (ester) and 2-fucosyllactose (2′-FL) as model products, whose production is hampered by cytosolic acidification. The engineered E. coli strains containing the proton-selective viroporin exhibited approximately 80% and 230% higher concentrations of the ester and 2′-FL, respectively, than the control strains without the M2 protein. The simple and powerful strategy developed in this study can be applied to produce other valuable chemicals whose production involves substrates and/or products that cause cytosolic acidification. © 2021 International Metabolic Engineering Society
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