Gold nanoparticle-assisted SELEX as a visual monitoring platform for the development of small molecule-binding DNA aptasensors
- 주제(키워드) Aptamers , Biosensor , Bisphenol , Brassinolide , Colorimetry , Gold nanoparticle , SELEX
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182149
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bios.2021.113468
- PubMed https://pubmed.ncbi.nlm.nih.gov/34233257
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
To resolve time-consuming and imperceptible monitoring problems in the traditional systematic evolution of ligands by exponential enrichment (SELEX), we report gold nanoparticle-assisted SELEX (GNP-SELEX) as a visual, proofreading, and self-monitoring platform and its application to small molecule-binding single-stranded DNA (ssDNA) aptasensors. Through the colorimetric changes between rounds, GNP-SELEX enabled the rapid determination of target-specific aptamer library enrichment with neither target modification nor extra monitoring process. We identified ssDNA aptamers with high selectivity and binding affinity by targeting two small molecules (brassinolide; BL and bisphenol A; BPA) as a model. The rational design of selected aptamers by 3D molecular simulation increased their ability to detect BL or BPA in real samples as bioreceptors. These results suggest that GNP-SELEX is useful as a self-monitoring platform to discover ssDNA aptamers as well as to develop aptasensors for diverse targets in a rapid and simple way. © 2021 Elsevier B.V.
more