Hypochlorite-Activated Fluorescence Emission and Antibacterial Activities of Imidazole Derivatives for Biological Applications
- 주제(키워드) antibacterial effect , fluorescent sensor , fluorogenic probe , hypochlorite sensor , probe–killer
- 등재 SCIE, SCOPUS
- 발행기관 Frontiers Media S.A.
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182174
- 본문언어 영어
- Published As http://dx.doi.org/10.3389/fchem.2021.713078
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The ability to detect hypochlorite (HOCl/ClO−) in vivo is of great importance to identify and visualize infection. Here, we report the use of imidazoline-2-thione (R1SR2) probes, which act to both sense ClO− and kill bacteria. The N2C=S moieties can recognize ClO− among various typical reactive oxygen species (ROS) and turn into imidazolium moieties (R1IR2) via desulfurization. This was observed through UV–vis absorption and fluorescence emission spectroscopy, with a high fluorescence emission quantum yield (ՓF = 43–99%) and large Stokes shift (∆v∼115 nm). Furthermore, the DIM probe, which was prepared by treating the DSM probe with ClO−, also displayed antibacterial efficacy toward not only Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) but also methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum ß-lactamase–producing Escherichia coli (ESBL-EC), that is, antibiotic-resistant bacteria. These results suggest that the DSM probe has great potential to carry out the dual roles of a fluorogenic probe and killer of bacteria. © Copyright © 2021 Pham, Nguyen, Choi, Kim, Jung, Lee, Kim, Lee, Yoon, Kim and Lee.
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