Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Cho, Seo Won; Lim, Hyun Jeong; Son, Ahjeong] Ewha Womans Univ, Dept Environm Sci & Engn, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Cho, Seo Won] Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA; [Chua, Beelee] Korea Univ, Sch Elect Engn, 145 Anam Ro, Seoul 02841, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ROYAL SOC CHEMISTRY
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182211
- 본문언어 영어
- Published As http://dx.doi.org/10.1039/d1ra00971k
초록/요약
The purpose of this study is to investigate the possibility of improving the performance of a DNA binding dye water quenching based aptasensor without changing or truncating the aptamer. To demonstrate the possibility of increasing the change in fluorescence of the aptasensor by pairing it with a suitable ssDNA probe, three ssDNA probes (probe 1, 2, and 3) were employed and the fluorescence from the bound dyes was measured. This showed that ssDNA probe 2 created the most additional binding sites. By varying the target compound concentration (0, 0.05, 0.5, 5, 50, and 500 mg L-1 4-n-nonylphenol), the corresponding change in the fluorescence signal of the unpaired and ssDNA probe paired aptasensors were measured and compared over a range of emission wavelengths. The response of all three ssDNA probe paired aptasensors showed good fit (R-2 = 0.88-0.92) to a logarithmic response. The sensitivity of the aptasensor paired with ssDNA probe 2 was improved by similar to 60%, whereas that of the aptasensor paired with ssDNA probe 3 was only improved by a marginal similar to 3%. This study is a demonstration of using an appropriate ssDNA probe to increase the number of binding sites and hence the performance of a DNA binding dye and water quenched aptasensor. It is a possibility that can be extended to similar aptasensors without having to change or truncate the aptamer.
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