Inhibitory activity of gintonin on inflammation in human IL-1 beta-stimulated fibroblast-like synoviocytes and collagen-induced arthritis in mice
- 주제(키워드) gintonin , arthritis , inflammation , collagen-induced arthritis , cytokines
- 주제(기타) Plant Sciences
- 주제(기타) Chemistry, Medicinal
- 주제(기타) Integrative & Complementary Medicine
- 설명문(일반) [Kim, Mijin; Sur, Bongjun; Villa, Thea; Oh, Seikwan] Ewha Womans Univ, Sch Med, Dept Mol Med, Seoul 07804, South Korea; [Nah, Seung Yeol] Konkuk Univ, Coll Vet Med, Ginsentol Res Lab, Seoul, South Korea; [Nah, Seung Yeol] Konkuk Univ, Coll Vet Med, Dept Physiol, Seoul, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN SOC GINSENG
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182243
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jgr.2020.12.001
초록/요약
Background: Gintonin is a newly derived glycolipoprotein from the roots of ginseng. The purpose of this study is to investigate the anti-arthritic efficacy of Gintonin on various proteases and inflammatory mediators that have an important role in arthritis. Methods: Fibroblast-like synoviocytes (FLS) were treated with Gintonin and stimulated with interleukin (IL)-1 beta 1 hour later. The antioxidant effect of Gintonin was measured using MitoSOX and H(2)DCFDA experiments. The anti-arthritic efficacy of Gintonin was examined by analyzing the expression levels of inflammatory mediators using RT-PCR, western blot, and ELISA. The phosphorylation of mitogen-activated protein kinase (MAPK) pathways and translocation of nuclear factor kappa B (NF-kappa B)/p65 into the nucleus were also analyzed using western blot, ELISA, and immunocytochemistry. Collagen-induced arthritis (CIA) mice model was used. Mice were orally administered with Gintonin (25, 50, and 100 mg/kg) every 2 days for 45 days. The body weight, arthritis score, squeaking score, and paw volume were measured as the behavioral parameters. After sacrifice, H&E and safranin-O staining were performed for histological analysis. Results: Gintonin significantly inhibited the expression of inflammatory intermediates. Gintonin prevented NF-kappa B/p65 from moving into the nucleus through the JNK and ERK MAPK phosphorylation in FLS cells. Moreover, Gintonin suppressed the symptoms of arthritis in the CIA mice model. Conclusion: As a result, the antioxidant and anti-inflammatory effects of Gintonin were demonstrated, and ultimately the anti-arthritic effect was proved. Collectively, Gintonin has a great potential as a therapeutic agent for arthritis treatment. (C) 2020 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
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