Identification of Indicators for Preterm Birth Using Retinoid Metabolites
- 주제(키워드) preterm birth , metabolomic analysis , retinoid metabolism , parturition , 13-cis-retinoic acid
- 주제(기타) Biochemistry & Molecular Biology
- 설명문(일반) [You, Young-Ah; Park, Sunwha; Ansari, AbuZar; Kim, Young-Ju] Ewha Womans Univ, Ewha Med Inst, Dept Obstet & Gynecol, Seoul 07804, South Korea; [You, Young-Ah; Park, Sunwha; Ansari, AbuZar; Kim, Young-Ju] Ewha Womans Univ, Coll Med, Seoul 07804, South Korea; [Hwang, Soo-Yeon; Park, Seojeong; Kwon, Youngjoo] Ewha Womans Univ, Dept Pharm, Seoul 03760, South Korea; [Kim, Soo-Min; Lee, Ga-In; Kim, Young-Ju] Ewha Womans Univ, Grad Program Syst Hlth Sci & Engn, Seoul 03760, South Korea; [Lee, Jeongae] Korea Inst Sci & Technol, MolecularRecognit Res Ctr, Seoul 02792, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 MDPI
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182304
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/metabo11070443
초록/요약
Metabolites reflect the biochemical dynamics for the maintenance of pregnancy and parturition. UPLC-Q/TOF-MS and LC-MS/MS metabolomics were performed to identify and validate the plasma metabolomic signatures of preterm birth (PTB). We recruited pregnant women between 16 and 40 weeks 5 days gestational age at Ewha Womans Mokdong Hospital for a nested case-control study. In untargeted UPLC-Q/TOF-MS, score plots of partial least-squares discriminant analysis clearly separated the PTB group from the term birth (TB, n = 10; PTB, n = 11). Fifteen metabolites were significantly different between the two groups, as indicated by a variable importance in projection >1 and p < 0.05. Metabolic pathways involving retinol, linoleic acid, d-arginine, and d-ornithine were associated with PTB. Verification by LC-MS/MS focused on retinol metabolism (TB, n = 39; PTB, n = 20). Retinol levels were significantly reduced in PTB compared to TB, while retinal palmitate, all-trans-retinal, and 13-cis-retinoic acid ((13)cis-RA) significantly increased (p < 0.05). Retinol-binding protein levels were also elevated in PTB. Additionally, all-trans-retinal (AUC 0.808, 95% CI: 0.683-0.933) and (13)cis-RA (AUC 0.826, 95% CI: 0.723-0.930) showed improved predictions for PTB-related retinol metabolites. This study suggests that retinoid metabolism improves the accuracy of PTB predictions and plays an important role in maintaining pregnancy and inducing early parturition.
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