Titanium dioxide nanoparticles enhance thrombosis through triggering the phosphatidylserine exposure and procoagulant activation of red blood cells
- 주제(키워드) Titanium dioxide nanoparticles (TiO2 NPs) , Phosphatidylserine (PS) exposure , Procoagulant activity , Thrombosis , Red blood cells (RBCs)
- 주제(기타) Toxicology
- 설명문(일반) [Bian, Yiying; Pi, Jingbo] China Med Univ, Sch Publ Hlth, Shenyang 110122, Peoples R China; [Chung, Han-Young] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151742, South Korea; [Chung, Han-Young] Seoul Natl Univ, Ctr Food Safety & Toxicol, Seoul 151742, South Korea; [Bae, Ok-Nam] Hanyang Univ, Coll Pharm, Ansan 426791, Gyeonggido, South Korea; [Lim, Kyung-Min] Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea; [Chung, Jin-Ho] Seoul Natl Univ, Coll Pharm, Seoul 151742, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 BMC
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182362
- 본문언어 영어
- Published As http://dx.doi.org/10.1186/s12989-021-00422-1
초록/요약
Background Expanding biomedical application of anatase titanium dioxide (TiO2) nanoparticles (NPs) is raising the public concern on its potential health hazards. Here, we demonstrated that TiO2 NPs can increase phosphatidylserine (PS) exposure and procoagulant activity of red blood cells (RBCs), which may contribute to thrombosis. Results We conducted in vitro studies using RBCs freshly isolated from healthy male volunteers. TiO2 NPs exposure (<= 25 mu g/mL) induced PS exposure and microvesicles (MV) generation accompanied by morphological changes of RBCs. While ROS generation was not observed following the exposure to TiO2 NPs, intracellular calcium increased and caspase-3 was activated, which up-regulated scramblase activity, leading to PS exposure. RBCs exposed to TiO2 NPs could increase procoagulant activity as measured by accelerated thrombin generation, and enhancement of RBC-endothelial cells adhesion and RBC-RBC aggregation. Confirming the procoagulant activation of RBC in vitro, exposure to TiO2 NPs (2 mg/kg intravenously injection) in rats increased thrombus formation in the venous thrombosis model. Conclusion Collectively, these results suggest that anatase TiO2 NPs may harbor prothrombotic risks by promoting the procoagulant activity of RBCs, which needs attention for its biomedical application.
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