A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells
- 주제(키워드) Estrogen , Tonsil , Mesenchymal stem cell , Secretion , Cytochrome P450 family 19 subfamily A member 1
- 주제(기타) Cell & Tissue Engineering
- 주제(기타) Engineering, Biomedical
- 설명문(일반) [Kim, Hee-Yeon; Lee, Younghay; Yoon, Hee-Soo; Jung, Sung-Chul; Park, Joo-Won] Ewha Womans Univ, Coll Med, Dept Biochem, Seoul 07804, South Korea; [Kim, Yu-Hee; Cho, Kyong-A; Woo, So-Youn] Ewha Womans Univ, Coll Med, Dept Microbiol, Seoul 07804, South Korea; [Kim, Han Sun] Ewha Womans Univ, Coll Med, Dept Otolaryngol, Seoul 07985, South Korea; [Park, Bo-Young] Ewha Womans Univ, Coll Med, Dept Plast & Reconstruct Surg, Seoul 07804, South Korea; [Jo, Inho] Ewha Womans Univ, Coll Med, Dept Mol Med, Seoul 07804, South Korea; [Park, Woo-Jae] Gachon Univ, Coll Med, Dept Biochem, Incheon 21999, South Korea; [Ryu, Kyung-Ha] Ewha Womans Univ, Coll Med, Dept Pediat, Seoul 07804, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published
- 발행기관 KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182438
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s13770-020-00307-y
- PubMed https://pubmed.ncbi.nlm.nih.gov/33113109
초록/요약
BACKGROUND: The advantages of tonsil-derived mesenchymal stem cells (TMSCs) over other mesenchymal stem cells (MSCs) include higher proliferation rates, various differentiation potentials, efficient immune-modulating capacity, and ease of obtainment. Specifically, TMSCs have been shown to differentiate into the endodermal lineage. Estrogen deficiency is a major cause of postmenopausal osteoporosis and is associated with higher incidences of ischemic heart disease and cerebrovascular attacks during the postmenopausal period. Therefore, stem cell-derived, estrogen-secreting cells might be used for estrogen deficiency. METHODS: Here, we developed a novel method that utilizes retinoic acid, insulin-like growth factor-1, basic fibroblast growth factor, and dexamethasone to evaluate the differentiating potential of TMSCs into estrogen-secreting cells. The efficacy of the novel differentiating method for generation of estrogen-secreting cells was also evaluated with bone marrow- and adipose tissue-derived MSCs. RESULTS: Incubating TMSCs in differentiating media induced the gene expression of cytochrome P450 19A1 (CYP19A1), which plays a key role in estrogen biosynthesis, and increased 17 beta-estradiol secretion upon testosterone addition. Furthermore, CYP11A1, CYP17A1, and 3 beta-hydroxysteroid dehydrogenase type-1 gene expression levels were significantly increased in TMSCs. In bone marrow-derived and adipose tissue-derived MSCs, this differentiation method also induced the gene expression of CYP19A1, but not CYP17A1, suggesting TMSCs are a superior source for estrogen secretion. CONCLUSION: These results imply that TMSCs can differentiate into functional estrogen-secreting cells, thus providing a novel, alternative cell therapy for estrogen deficiency.
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