Characterization of humoral and cellular immune features of gamma-irradiated influenza vaccine
- 주제(키워드) Influenza A virus , gamma-irradiation , whole cell inactivated vaccine , mucosal immunity , dendritic cells
- 주제(기타) Biotechnology & Applied Microbiology
- 주제(기타) Immunology
- 설명문(일반) [Chen, Fengjia; Seo, Ho Seong; Ji, Hyun Jung; Lim, Sangyong; Ahn, Ki Bum] Korea Atom Energy Res Inst, Radiat Res Div, Jeongeup, South Korea; [Seo, Ho Seong; Lim, Sangyong] Univ Sci & Technol, Dept Radiat Biotechnol & Appl Radioisotope Sci, Daejeon, South Korea; [Ji, Hyun Jung; Han, Seung Hyun] Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, DRI, Seoul, South Korea; [Ji, Hyun Jung; Han, Seung Hyun] Seoul Natl Univ, Sch Dent, BK21 Plus Program, Seoul, South Korea; [Yang, Eunji; Choi, Jung Ah; Yang, Jae Seung; Song, Manki] Int Vaccine Inst, Sci Unit, Clin Res Lab, Seoul, South Korea; [Lim, Jae Hyang] Ewha Womans Univ, Dept Microbiol, Coll Med, Seoul 07804, South Korea; [Lim, Jae Hyang] Ewha Womans Univ, Ewha Educ & Res Ctr Infect, Med Ctr, Seoul, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published, Bronze
- 발행기관 TAYLOR & FRANCIS INC
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000182493
- 본문언어 영어
- Published As http://dx.doi.org/10.1080/21645515.2020.1780091
- PubMed https://pubmed.ncbi.nlm.nih.gov/32643515
초록/요약
The most widely used influenza vaccines are prepared by chemical inactivation. However, chemical, especially formalin, treatment-induced modifications of the antigenic structure of the virus are frequently associated with adverse effects including low efficacy of protection, unexpected immune responses, or exacerbation of disease. Gamma-irradiation was suggested as an alternative influenza virus inactivation method due to its great features of completely inactivating virus while not damaging the structures of protein antigens, and cross-protective ability against heterologous strains. However, immunological features of gamma radiation-inactivated influenza vaccine have not been fully understood. In this study, we aimed to investigate the humoral and cellular immune responses of gamma radiation-inactivated influenza vaccine. The gamma irradiation-inactivated influenza vaccine (RADVAX(FluA)) showed complete viral inactivation but retained normal viral structure with functional activities of viral protein antigens. Intranasal immunization of RADVAX(FluA)provided better protection against influenza virus infection than formalin-inactivated influenza virus (FIV) in mice. RADVAX(FluA)greatly enhanced the production of virus-specific serum IgG and alveolar mucosal IgA, which effectively neutralized HA (hemagglutinin) and NA (neuraminidase) activities, and blocked viral binding to the cells, respectively. Further analysis of IgG subclasses showed RADVAX(FluA)-immunized sera had higher levels of IgG1 and IgG2a than those of FIV-immunized sera. In addition, analysis of cellular immunity found RADVAX(FluA)induced strong dendritic cells (DC) activation resulting in higher DC-mediated activation of CD8(+)T cells than FIV. The results support improved immunogenicity by RADVAX(FluA).
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