Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors: A Fatty Acid Conversion Case Study
- 주제(키워드) biocatalysis , decarboxylase , hydratase , nano-scale bioreactor , outer membrane vesicles
- 등재 SCIE, SCOPUS
- 발행기관 John Wiley and Sons Inc
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183296
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/cctc.202100778
초록/요약
Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV. © 2021 The Authors. ChemCatChem published by Wiley-VCH GmbH
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