Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors
- 주제(키워드) mesenchymal stem cell , exosomes , skeletal muscle , activin A
- 주제(기타) Cell Biology
- 설명문(일반) [Cho, Kyung-Ah; Choi, Da-Won; Kim, Jungwoo; Woo, So-Youn] Ewha Womans Univ, Coll Med, Dept Microbiol, Seoul 07804, South Korea; [Choi, Da-Won] Ewha Womans Univ, Syst Biohlth BK21, Seoul 07804, South Korea; [Kim, Yu-Hee] Ewha Womans Univ, Adv Biomed Res Inst, Seoul Hosp, Seoul 07804, South Korea; [Ryu, Kyung-Ha] Ewha Womans Univ, Coll Med, Dept Pediat, Seoul 07804, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 MDPI
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183470
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/cells10082169
초록/요약
Skeletal muscle mass is decreased under a wide range of pathologic conditions. In particular, chemotherapy is well known for inducing muscle loss and atrophy. Previous studies using tonsil-derived mesenchymal stem cells (T-MSCs) or a T-MSC-conditioned medium showed effective recovery of total body weight in the chemotherapy-preconditioned bone marrow transplantation mouse model. This study investigated whether extracellular vesicles of T-MSCs, such as exosomes, are a key player in the recovery of body weight and skeletal muscle mass in chemotherapy-treated mice. T-MSC exosomes transplantation significantly decreased loss of total body weight and muscle mass in the busulfan-cyclophosphamide conditioning regimen in BALB/c recipient mice containing elevated serum activin A. Additionally, T-MSC exosomes rescued impaired C2C12 cell differentiation in the presence of activin A in vitro. We found that T-MSC exosomes possess abundant miR-145-5p, which targets activin A receptors, ACVR2A, and ACVR1B. Indeed, T-MSC exosomes rescue muscle atrophy both in vivo and in vitro via miR-145-5p dependent manner. These results suggest that T-MSC exosomes have therapeutic potential to maintain or improve skeletal muscle mass in various activin A elevated pathologic conditions.
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