Development of a Human Estrogen Receptor Dimerization Assay for the Estrogenic Endocrine-Disrupting Chemicals Using Bioluminescence Resonance Energy Transfer
- 주제(키워드) estrogenic endocrine-disrupting chemical , estrogen receptor , bioluminescence resonance energy transfer , risk assessment
- 주제(기타) Environmental Sciences; Public, Environmental & Occupational Health
- 설명문(일반) [Kim, Hye Mi; Ko, Kwang Suk] Ewha Womans Univ, Dept Nutr Sci & Food Management, Seoul 03760, South Korea; [Seo, Hyeyeong] Korea Univ, Dept Integrated Biomed & Life Sci, Seoul 02841, South Korea; [Park, Yooheon; Lee, Seok-Hee] Dongguk Univ, Dept Food Sci & Biotechnol, Goyang 10326, South Korea; [Lee, Hee-Seok] Chung Ang Univ, Dept Food Sci & Technol, Anseong 17546, South Korea
- 등재 SCIE, SSCI, SCOPUS
- 발행기관 MDPI
- 발행년도 2021
- URI http://www.dcollection.net/handler/ewha/000000183477
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/ijerph18168875
초록/요약
Endocrine-disrupting chemicals (EDCs) are found in food and various other substances, including pesticides and plastics. EDCs are easily absorbed into the body and have the ability to mimic or block hormone function. The radioligand binding assay based on the estrogen receptors binding affinity is widely used to detect estrogenic EDCs but is limited to radioactive substances and requires specific conditions. As an alternative, we developed a human cell-based dimerization assay for detecting EDC-mediated ER-alpha (ER alpha) dimerization using bioluminescence resonance energy transfer (BRET). The resultant novel BRET-based on the ER alpha dimerization assay was used to identify the binding affinity of 17 beta-estradiol (E2), 17 alpha-estradiol, corticosterone, diethylhexyl phthalate, bisphenol A, and 4-nonylphenol with ER alpha by measuring the corresponding BRET signals. Consequently, the BRET signals from five chemicals except corticosterone showed a dose-dependent sigmoidal curve for ER alpha, and these chemicals were suggested as positive chemicals for ER alpha. In contrast, corticosterone, which induced a BRET signal comparable to that of the vehicle control, was suggested as a negative chemical for ER alpha. Therefore, these results were consistent with the results of the existing binding assay for ER alpha and suggested that a novel BRET system can provide information about EDCs-mediated dimerization to ER alpha.
more