Fluorescence Probe for Imaging N-Methyl-D-aspartate Receptors and Monitoring GSH Selectively Using Two-Photon Microscopy
- 주제(기타) Chemistry, Analytical
- 설명문(일반) [Kwon, Nahyun; Ko, Gyeongju; Ha, Jeongsun; Lee, Dayoung; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03760, South Korea; [Lim, Chang Su; Kim, Hwan Myung] Ajou Univ, Dept Energy Syst Res, Suwon 443749, South Korea; [Yin, Jun] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China; [Yin, Jun] Cent China Normal Univ, Hubei Int Sci & Technol Cooperat Base Pesticide &, Wuhan 430079, Peoples R China; [Yin, Jun] Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensing Technol, Wuhan 430079, Peoples R China; [Yin, Jun] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183494
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.analchem.1c02350
초록/요약
N-Methyl-D-aspartate (NMDA) is an excitotoxic amino acid used to identify a specific subset of glutamate receptors. The activity of NMDA receptors is closely related to the redox level of the biological system. Glutathione (GSH) as an antioxidant plays a key role with regard to modulation of the redox environment. In this work we designed and developed a GSH-specific fluorescent probe with the capability of targeting NMDA receptors, which was composed of a two-photon naphthalimide fluorophore, a GSHreactive group sulfonamide, and an ifenprodil targeting group for the NMDA receptor. This probe exhibited high selectivity toward GSH in comparison to other similar amino acids. Two-photon fluorescence microscopy allowed this probe to successfully monitor GSH in neuronal cells and hippocampal tissues with an excitation at 750 nm. It could serve as a potential practical imaging tool to explore the function of GSH and related biological processes in the brain.
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