Comparative Study of the Difference in Behavior of the Accessory Gene Regulator (Agr) in USA300 and USA400 Community- Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA)
- 주제(키워드) CA-MRSA , USA300 , USA400 , function of Agr system , strain-dependent behaviors
- 주제(기타) Biotechnology & Applied Microbiology; Microbiology
- 설명문(일반) [Lee, Hye Soo; Song, Hun-Suk; Lee, Hong-Ju; Kim, Sang Hyun; Suh, Min Ju; Cho, Jang Yeon; Ham, Sion; Bhatia, Shashi Kant; Yang, Yung-Hun] Konkuk Univ, Dept Biol Engn, Coll Engn, Seoul 05029, South Korea; [Kim, Yun-Gon] Soongsil Univ, Dept Chem Engn, Seoul 07040, South Korea; [Joo, Hwang-Soo] Duksung Womens Univ, Dept Biotechnol, Coll Engn, Seoul 01369, South Korea; [Kim, Wooseong] Ewha Womans Univ, Coll Pharm, Seoul 03760, South Korea; [Kim, Wooseong] Ewha Womans Univ, Grad Sch Pharmaceut Sci, Seoul 03760, South Korea; [Lee, Sang Ho] Jeju Natl Univ, Dept Pharm, Coll Pharm, Jeju 63243, South Korea; [Yoo, Dongwon] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 08826, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183496
- 본문언어 영어
- Published As http://dx.doi.org/10.4014/jmb.2104.04032
초록/요약
Community-associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) is notorious as a leading cause of soft tissue infections. Despite several studies on the Agr regulator, the mechanisms of action of Agr on the virulence factors in different strains are still unknown. To reveal the role of Agr in different CA-MRSA, we investigated the LAC Delta agr mutant and the MW2 Delta agr mutant by comparing LAC (USA300), MW2 (USA400), and Delta agr mutants. The changes of Delta agr mutants in sensitivity to oxacillin and several virulence factors such as biofilm formation, pigmentation, motility, and membrane properties were monitored. LAC Delta agr and MW2 Delta agr mutants showed different oxacillin sensitivity and biofilm formation compared to the LAC and MW2 strains. Regardless of the strain, the motility was reduced in Delta agr mutants. And there was an increase in the long chain fatty acid in phospholipid fatty acid composition of Delta agr mutants. Other properties such as biofilm formation, pigmentation, motility, and membrane properties were different in both Delta agr mutants. The Agr regulator may have a common role like the control of motility and strain dependent roles such as antibiotic resistance, biofilm formation, change of membrane, and pigment production. It does not seem easy to control all MRSA by targeting the Agr regulator only as it showed strain-dependent behaviors.
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