A "Prime and Deploy" Strategy for Universal Influenza Vaccine Targeting Nucleoprotein Induces Lung-Resident Memory CD8 T cells
- 주제(키워드) Lung CD8 T-RM , DNA vaccine , Adenovirus vector , Intranasal , Influenza B virus
- 주제(기타) Immunology
- 설명문(일반) [Chung, Haerynn; Kim, Eun-Ah; Chang, Jun] Ewha Womans Univ, Grad Sch Pharmaceut Sci, 52 Ewhayeodae Gil, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREA ASSOC IMMUNOLOGISTS
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183497
- 본문언어 영어
- Published As http://dx.doi.org/10.4110/in.2021.21.e28
초록/요약
Lung-resident memory T cells (T-RM) play an essential role in protecting against pulmonary virus infection. Parenteral administration of DNA vaccine is generally not sufficient to induce lung CD8 T-RM cells. This study investigates whether intramuscularly administered DNA vaccine expressing the nucleoprotein (NP) induces lung T-RM cells and protects against the influenza B virus. The results show that DNA vaccination poorly generates lung T-RM cells and massive secondary effector CD8 T cells entering the lungs after challenge infection do not offer sufficient protection. Nonetheless, intranasal administration of non-replicating adenovirus vector expressing no Ag following priming DNA vaccination deploys NP-specific CD8T T-RM cells in the lungs, which subsequently offers complete protection. This novel 'prime and deploy' strategy could be a promising regimen for a universal influenza vaccine targeting the conserved NP Ag.
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