Reasonably constructed NIR fluorescent probes based on dicyanoisophorone skeleton for imaging ONOO- in living cells
- 주제(키워드) Reactive nitrogen species , pKa regulation , Cell imaging , NIR Fluorescent probe
- 주제(기타) Chemistry, Applied; Engineering, Chemical; Materials Science, Textiles
- 설명문(일반) [Chung, Jeewon; Kim, Heejeong; Li, Haidong; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCI LTD
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183520
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.dyepig.2021.109665
초록/요약
Peroxynitrite (ONOO-), a reactive nitrogen species (RNS), engages in various cellular processes including inflammation, apoptosis, and necrosis. Therefore, the development of a powerful tool for imaging endogenous ONOO- is important with regard to revealing its underlying physiological or pathological functions. In this work, we introduced dihalogen (Cl, Br, and I) at the ortho-positions of the hydroxyl group of a dicyanoisophorone skeleton to adjust its pKa to be more suitable for bio-imaging in the physiological environments with a high signal-to-noise ratio. Based on the optimized spectral results and the requirement of biocompatibility, the fluorescent probe NIR-dCl was successfully designed and fabricated for the detection of ONOO-. Upon the addition of ONOO- in solution, an obvious NIR fluorescent signal at 680 nm was observed. Moreover, the probe NIR-dCl exhibited high selectivity for sensing ONOO-. Cell imaging results confirmed that probe NIR-dCl was capable of imaging exogenous and endogenous ONOO- in live cells. Thus, we believe that probe NIR-dCl could be an effective tool for studying biochemical processes and ONOO--related diseases.
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