Toll-Like Receptor 7 (TLR7) Mediated Transcriptomic Changes on Human Mast Cells
- 주제(키워드) Mast cell , Skin inflammation , Toll-like receptor 7
- 주제(기타) Dermatology
- 설명문(일반) [Cho, Kyung-Ah; Choi, Da-Won; Park, Minhwa; Kim, Yu-Hee; Woo, So-Youn] Ewha Womans Univ, Coll Med, Dept Microbiol, 25 Magokdong Ro 2 Gil, Seoul 07804, South Korea; [Kim, Yu-Hee] Ewha Womans Univ, Seoul Hosp, Adv Biomed Res Inst, 260 Gonghang Daero, Seoul 07804, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published, gold
- 발행기관 KOREAN DERMATOLOGICAL ASSOC
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183652
- 본문언어 영어
- Published As http://dx.doi.org/10.5021/ad.2021.33.5.402
- PubMed https://pubmed.ncbi.nlm.nih.gov/34616120
초록/요약
Background: Mast cells are skin immune sentinels located in the upper dermis, where wheal formation and sensory nerve stimulation take place. Skin inflammation is occasionally accompanied by mast cell-driven responses with wheals, angioedema, or both. Immunoglobulin E (IgE) antibodies are regarded as typical stimuli to drive mast cell activation. However, various causative factors, including microbial infections, can drive IgE-independent mast cell response. When infected, the innate immunity orchestrates an immune response by activating receptor signaling via Toll-like receptors (TLRs). Objective: In this study, we determined the effect of TLR7 stimulation on mast cells to investigate the possible mechanism of IgE-independent inflammatory response. Methods: Human mast cell (HMC) line, HMC-1 cells were treated with TLR7 agonist and the morphologic alteration was observed in transmission electron microscopy. Further, TLR7 agonist treated HMC-1 cells were conducted to RNA sequencing to compare transcriptomic features. Results: HMC-1 cells treated with TLR7 agonist reveals increase of intracellular vesicles, lipid droplets, and ribosomes. Also, genes involved in pro-inflammatory responses such as angiogenesis are highly expressed, and Il12rb2 was the most highly up regulated gene. Conclusion: Our data suggest that TLR7 signaling on mast cells might be a potential therapeutic target for mast cell-driven, IgE-independent skin inflammation. (Ann Dermatol 33(5) 402 similar to 408, 2021)
more