Two-photon ESIPT-based fluorescent probe using 4-hydroxyisoindoline-1,3-dione for the detection of peroxynitrite
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Wu, Luling; Tian, Xue; James, Tony D.] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England; [Lee, Dong Joon; Lim, Chang Su; Kim, Hwan Myung] Ajou Univ, Dept Energy Syst Res, Suwon 443749, South Korea; [Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 03760, South Korea; [James, Tony D.] Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Henan, Peoples R China
- 등재 SCIE, SCOPUS
- OA유형 hybrid
- 발행기관 ROYAL SOC CHEMISTRY
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183659
- 본문언어 영어
- Published As http://dx.doi.org/10.1039/d1cc03160k
- PubMed https://pubmed.ncbi.nlm.nih.gov/34617087
초록/요약
Excited-state intramolecular proton transfer (ESIPT)-based fluorophores with two-photon excitation fluorescence (TPEF) are rare. Our aim with this research was to develop ESIPT-based fluorophores exhibiting TPEF. Herein, we used 4-hydroxyisoindoline-1,3-dione as a scaffold to develop a two-photon fluorescent probe BHID-Bpin, for the detection of peroxynitrite (ONOO-). BHID-Bpin exhibits excellent selectivity, sensitivity, and fast response towards ONOO- in PBS buffer solution (10 mM, pH = 7.40). Additionally, BHID-Bpin displays high photo-stability under two-photon irradiation at 750 nm. Furthermore, the probe can image endogenous ONOO- in HeLa cells and exogenous ONOO- in rat hippocampal slices at a depth of 110 mu m.
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