Extracellular vesicles from tonsil-derived mesenchymal stromal cells show anti-tumor effect via miR-199a-3p
- 주제(키워드) tonsil-derived mesenchymal stromal cells , microRNA , exosome , tumor suppression
- 주제(기타) Medicine, Research & Experimental
- 설명문(일반) [Choi, Da-Won; Cho, Kyung-Ah; Kim, Jungwoo; Lee, Hyun-Ji; Kim, Yu-Hee; Woo, So-Youn] Ewha Womans Univ, Dept Microbiol, Coll Med, 25 Magokdong Ro 2 Gil, Seoul 07804, South Korea; [Choi, Da-Won] Ewha Womans Univ, Syst Biohlth Brain Korea 21, Seoul 07804, South Korea; [Park, Jang-Won] Ewha Womans Univ, Dept Orthopaed Surg, Coll Med, Seoul 07804, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published, hybrid
- 발행기관 SPANDIDOS PUBL LTD
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183808
- 본문언어 영어
- Published As http://dx.doi.org/10.3892/ijmm.2021.5054
- PubMed https://pubmed.ncbi.nlm.nih.gov/34676871
초록/요약
Mesenchymal stem cells (MSCs) are mesoderm-originated adult SCs that possess multidirectional differentiation potential. MSCs migrate to injured tissue and secrete a range of paracrine factors that induce regeneration in damaged tissue and exert immune modulation. Because tumor progression is dependent on cross-talk between the tumor and its microenvironment, MSCs also produce extra-cellular vesicles (EVs) that mediate information transfer in the tumor microenvironment. However, the effect of MSC-derived EVs on tumor development and progression is still controversial. To date, tonsil-derived MSCs (T-MSCs) have been shown to possess all the defined characteristics of MSCs and show distinctive features of differential potential and immune modulation. To observe the effect of soluble mediators from T-MSCs on tumor growth, human liver cancer cell line (HepG2) cells were injected into nude mice and HepG2 cell scratch migration assay was performed using conditioned medium (CM) of T-MSCs. T-MSC CM inhibited tumor growth and progression and it was hypothesized that EVs from T-MSCs could inhibit tumor progression. microRNA (miRNA or miR) sequencing using five different origins of T-MSC-derived EVs was performed and highly expressed miRNAs, such as miR-199a-3p, miR-214-3p, miR-199a-5p and miR-199b-5p, were selected. T-MSCs inhibited tumor growth and HepG2 cell migration, potentially via miR-199a-3p targeting CD151, integrin alpha 3 and 6 in HepG2 cells.
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