Extracellular Vesicles from Korean Codium fragile and Sargassum fusiforme Negatively Regulate Melanin Synthesis
- 주제(키워드) extracellular vesicles , melanin synthesis , seaweed , skin epidermis
- 주제(기타) Biochemistry & Molecular Biology; Cell Biology
- 설명문(일반) [Jang, Bohee; Chung, Heesung; Jung, Hyejung; Song, Hyun-Kuk; Park, Eunhye; Oh, Eok-Soo] Ewha Womans Univ, Res Ctr Cellular Homeostasis, Dept Life Sci, Seoul 03760, South Korea; [Choi, Hack Sun] Jeju Natl Univ, Subtrop Trop Organism Gene Bank, Jeju 63243, South Korea; [Jung, Kyuhyun; Yang, Sanghwa] ExoMed Inc, Seoul 01795, South Korea; [Choe, Han] Univ Ulsan, Asan Med Ctr, Dept Physiol, Coll Med, Seoul 05505, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published, gold
- 발행기관 KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000183818
- 본문언어 영어
- Published As http://dx.doi.org/10.14348/molcells.2021.2167
- PubMed https://pubmed.ncbi.nlm.nih.gov/34650007
초록/요약
Although various marine ingredients have been exploited for the development of cosmetic products, no previous study has examined the potential of seaweed extracellular vesicles (EV) in such applications. Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased alpha-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, associated with downregulation of MITF (microphthalmia-associated transcription factor), tyrosinase and TRP1 (tyrosinase-related proteins 1). The most effective inhibitory concentrations of EV were 250 mu g/ml for S. fusiforme and 25 mu g/mlfor C. fragile, without affecting the viability of MNT-1 cells. Both EV reduced melanin synthesis in the epidermal basal layer of a three-dimensional model of human epidermis. Moreover, the application of the prototype cream containing C. fragile EV (final 5 mu g/ml) yielded 1.31 % improvement in skin brightness in a clinical trial. Together, these results suggest that EV from C. fragile and S. fusiforme reduce melanin synthesis and may be potential therapeutic and/or supplementary whitening agents.
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