A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate
- 주제(키워드) Liver fibrosis , Polyhexamethylene guanidine phosphate (PHMG-p) , Murine liver fibrosis model , Lumican , IRAK3
- 주제(기타) Biochemistry & Molecular Biology
- 주제(기타) Pharmacology & Pharmacy
- 설명문(일반) [Kim, Minjeong; Lim, Kyung-Min] Ewha Womans Univ, Coll Pharm, Seoul 03760, South Korea; [Hur, Sumin; Kim, Kwang H.; Cho, Yejin; Nam, Ki Taek] Yonsei Univ, Coll Med, Severance Biomed Sci Inst, Brain Korea 21 PLUS Project Med Sci, Seoul 03722, South Korea; [Kim, Keunyoung] Kangwon Natl Univ, Coll Pharm, Chunchon 24341, South Korea; [Kim, Ha Ryong] Daegu Catholic Univ, Coll Pharm, Daegu 38430, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published, Green Submitted, hybrid
- 발행기관 KOREAN SOC APPLIED PHARMACOLOGY
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000190453
- 본문언어 영어
- Published As https://doi.org/10.4062/biomolther.2021.120
- PubMed https://pubmed.ncbi.nlm.nih.gov/34580237
초록/요약
Liver fibrosis is part of the wound healing process to help the liver recover from the injuries caused by various liver-damaging insults. However, liver fibrosis often progresses to life-threatening cirrhosis and hepatocellular carcinoma. To overcome the limitations of current in vivo liver fibrosis models for studying the pathophysiology of liver fibrosis and establishing effective treatment strategies, we developed a new mouse model of liver fibrosis using polyhexamethylene guanidine phosphate (PHMG-p), a humidifier sterilizer known to induce lung fibrosis in humans. Male C57/BL6 mice were intraperitoneally injected with PHMG-p (0.03% and 0.1%) twice a week for 5 weeks. Subsequently, liver tissues were examined histologically and RNA-sequencing was performed to evaluate the expression of key genes and pathways affected by PHMG-p. PHMG-p injection resulted in body weight loss of similar to 15% and worsening of physical condition. Necropsy revealed diffuse fibrotic lesions in the liver with no effect on the lungs. Histology, collagen staining, immunohistochemistry for smooth muscle actin and collagen, and polymerase chain reaction analysis of fibrotic genes revealed that PHMG-p induced liver fibrosis in the peri-central, peri-portal, and capsule regions. RNA-sequencing revealed that PHMG-p affected several pathways associated with human liver fibrosis, especially with upregulation of lumican and IRAK3, and downregulation of GSTp1 and GSTp2, which are closely involved in liver fibrosis pathogenesis. Collectively we demonstrated that the PHMG-p-induced liver fibrosis model can be employed to study human liver fibrosis.
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