Generation of Recombinant Antibodies in HEK293F Cells for the Detection of Staphylococcus aureus
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 American Chemical Society
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000190475
- 본문언어 영어
- Published As https://doi.org/10.1021/acsomega.1c07194
- PubMed https://pubmed.ncbi.nlm.nih.gov/35350310
초록/요약
Staphylococcus aureus is a major resistant pathogen in clinical practice. Due to the increasing number of infections, rapid and sensitive detection of antibiotic-resistant S. aureus as well as antibiotic-sensitive S. aureus is important for the prevention and control of infectious diseases. In this study, we produced recombinant antibodies against S. aureus from mammalian human embryonic kidney 293 Freestyle cells with high yield and purity. These recombinant antibodies showed high binding affinity and low detection limit in both indirect and sandwich enzyme-linked immunosorbent assays for the detection of methicillin-resistant S. aureus and methicillin-sensitive S. aureus. These results suggest that the recombinant antibodies produced herein can be used for the accurate detection of S. aureus with a wild range of applications in medical diagnosis, food safety, and drug discovery. © 2022 The Authors. Published by American Chemical Society.
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