Improved Productivity of Naringin Oleate with Flavonoid and Fatty Acid by Efficient Enzymatic Esterification
- 주제(키워드) antioxidant , flavonoid , naringin , flavonoid ester , flavonoid oleate , lipase , esterification , enzymatic synthesis , optimization
- 주제(기타) Biochemistry & Molecular Biology
- 주제(기타) Chemistry, Medicinal
- 주제(기타) Food Science & Technology
- 설명문(일반) [Lee, Jonghwa; Kim, Kyeonga; Son, Jemin; Lee, Hyeseon; Song, Jin Han; Lee, Taek; Yoo, Hah Young; Park, Chulhwan] Kwangwoon Univ, Dept Chem Engn, Seoul 01897, South Korea; [Jeon, Heungbae] Kwangwoon Univ, Dept Chem, Seoul 01897, South Korea; [Kim, Hyun Soo] Kwangwoon Univ, Dept Elect Engn, Seoul 01897, South Korea; [Park, Si Jae] Ewha Womans Univ, Div Chem Engn & Mat Sci, Seoul 03760, South Korea; [Yoo, Hah Young] Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 MDPI
- 발행년도 2022
- URI http://www.dcollection.net/handler/ewha/000000191109
- 본문언어 영어
- Published As https://doi.org/10.3390/antiox11020242
- PubMed https://pubmed.ncbi.nlm.nih.gov/35204125
초록/요약
Naringin is a flavonoid found in citrus fruits. It exhibits biological activities, such as anticancer and antioxidant effects, but it suffers from low solubility and low stability in lipophilic systems. These drawbacks lead to difficulties in the commercial application of naringin, but they can be overcome through esterification. In this study, naringin oleate was synthesized by enzymatic esterification and optimal conditions for the reaction were investigated. Experiments were conducted focusing on the following parameters: enzyme type, enzyme concentration, molar ratio of naringin to oleic acid, reaction temperature, and reaction solvent. We further confirmed the degree of esterification based on the difference in the initial and the final naringin concentrations. A conversion of 93.10% was obtained under optimized conditions (Lipozyme TL IM 10 g/L, molar ratio 1:20, reaction temperature 40 degrees C, acetonitrile as solvent, and 48 h reaction time). Thus, naringin oleate, a high value-added material that overcomes the low hydrophobicity of naringin and enhances its performance, was obtained through esterification of naringin using oleic acid. This study presented a method for the efficient enzymatic synthesis that could ensure high conversion within a shorter reaction time compared with that required in previously reported methods.
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