Microbial production of 2-pyrone-4,6-dicarboxylic acid from lignin derivatives in an engineered Pseudomonas putida and its application for the synthesis of bio-based polyester
- 주제(키워드) 2-pyrone-4 , 6-dicarboxylic acid , Bio-based polyester , Biological funneling , Lignin , Pseudomonas putida
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- OA유형 hybrid
- 발행기관 Elsevier Ltd
- 발행년도 2022
- URI http://www.dcollection.net/handler/ewha/000000191124
- 본문언어 영어
- Published As https://doi.org/10.1016/j.biortech.2022.127106
- PubMed https://pubmed.ncbi.nlm.nih.gov/35378283
초록/요약
Lignin valorization depends on microbial upcycling of various aromatic compounds in the form of a complex mixture, including p-coumaric acid and ferulic acid. In this study, an engineered Pseudomonas putida strain utilizing lignin-derived monomeric compounds via biological funneling was developed to produce 2-pyrone-4,6-dicarboxylic acid (PDC), which has been considered a promising building block for bioplastics. The biosynthetic pathway for PDC production was established by introducing the heterologous ligABC genes under the promoter Ptac in a strain lacking pcaGH genes to accumulate a precursor of PDC, i.e., protocatechuic acid. Based on the culture optimization, fed-batch fermentation of the final strain resulted in 22.7 g/L PDC with a molar yield of 1.0 mol/mol and productivity of 0.21 g/L/h. Subsequent purification of PDC at high purity was successfully implemented, which was consequently applied for the novel polyester. © 2022 The Authors
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