Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
- 주제(키워드) <p>Pseudoalteromonas sp.</p> , pseudoalteromone A , marine natural product , melanogenesis
- 주제(기타) Chemistry, Medicinal
- 주제(기타) Pharmacology & Pharmacy
- 설명문(일반) [Lim, Su-Jin; Lee, Chang-Seok] Eulji Univ, Dept Beauty & Cosmet Sci, Seongnam 13135, South Korea; [Min, Dae-Jin; Lee, Eun-Soo; Kim, Hyuk; Cho, Sung-Yoen; Beak, Heung-Soo; Ko, Jaeyoung] Amorepacif R&D Unit, Basic Res & Innovat Div, Yongin 17074, South Korea; [Kim, Sohee] Ewha Womans Univ, Coll Pharm, Grad Sch Ind Pharmaceut Sci, Seoul 03760, South Korea; [Lee, Jihye; Nam, Sang-Jip] Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03760, South Korea; [Lee, Jihye] REDONE Seoul, Labs Marine New Drugs, Seoul 08594, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 MDPI
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000191136
- 본문언어 영어
- Published As https://doi.org/10.3390/md19110612
- PubMed https://pubmed.ncbi.nlm.nih.gov/34822483
초록/요약
An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 mu g/mL) for 72 h reduced alpha-melanocyte-stimulating hormone (alpha-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes.
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