A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-D-aspartate (NMDA) receptors
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Kwon, Nahyun; Lee, Dayoung; Ko, Gyeongju; Ha, Jeongsun; Cho, Moonyeon; Swamy, K. M. K.; Lee, Eun-Young; Nam, Sang-Jip; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03760, South Korea; [Lim, Chang Su; Lee, Dong Joon; Kim, Hwan Myung] Ajou Univ, Dept Energy Syst Res, Suwon 16499, South Korea; [Swamy, K. M. K.] VL Coll Pharm, Dept Pharmaceut Chem, Raichur 584103, India; [Zhou, Xin] Qingdao Univ, Dept Chem, Coll Chem & Chem Engn, Qingdao 266071, Shandong, Peoples R China
- 등재 SCIE, SCOPUS
- 발행기관 ROYAL SOC CHEMISTRY
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000191168
- 본문언어 영어
- Published As https://doi.org/10.1039/d1cc05512g
- PubMed https://pubmed.ncbi.nlm.nih.gov/35202451
초록/요약
Glutathione (GSH) is known to play a key role in the modulation of the redox environment in N-methyl-d-aspartate (NMDA) receptors. Coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties was synthesized and reported to monitor GSH near NMDA receptors. The cyanoacrylamide moiety allows probe 1 to monitor GSH reversibly at pH 7.4 and the ifenprodil group acts as a directing group for NMDA receptors. Two-photon fluorescence microscopy allows probe 1 to successfully sense endogenous GSH in neuronal cells and hippocampal tissues with excitation at 750 nm. Furthermore, the addition of H2O2 and GSH induced a decrease and an increase in fluorescence emission. Probe 1 can serve as a potential practical imaging tool to get important information on GSH in the brain.
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