Developing Microbial Co-Culture System for Enhanced Polyhydroxyalkanoates (PHA) Production Using Acid Pretreated Lignocellulosic Biomass
- 주제(키워드) Lysinibacillus sp , RGS , Ralstonia eutropha ATCC 17699 , co-culture strategy , sugarcane bagasse , acid pretreatment , polyhydroxyalkanoates production
- 주제(기타) Polymer Science
- 설명문(일반) [Saratale, Rijuta Ganesh; Kadam, Avinash Ashok] Dongguk Univ, Res Inst Biotechnol & Med Converged Sci, Goyang Si 10326, Gyeonggi Do, South Korea; [Cho, Si-Kyung; Ghodake, Gajanan Sampatrao] Dongguk Univ, Dept Biol & Environm Sci, Goyang Si 10326, Gyeonggi Do, South Korea; [Kumar, Manu] Dongguk Univ, Dept Life Sci, 32 Dongguk Ro, Goyang Si 10326, Gyeonggi Do, South Korea; [Bharagava, Ram Naresh] Ambedkar Univ, Sch Environm Sci Babasaheb Bhimrao, Dept Environm Microbiol, Lucknow 226025, Uttar Pradesh, India; [Varjani, Sunita] Gujarat Pollut Control Board, Gandhinagar 382010, Gujarat, India; [Nair, Supriya] SRL Ltd, Dept Res & Dev, Prime Sq,S V Rd, Mumbai 400062, Maharashtra, India; [Kim, Dong-Su] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 120750, South Korea; [Shin, Han-Seung; Saratale, Ganesh Dattatraya] Dongguk Univ, Dept Food Sci & Biotechnol, Goyang Si 10326, Gyeonggi Do, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 MDPI
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000191244
- 본문언어 영어
- Published As https://doi.org/10.3390/polym14040726
- PubMed https://pubmed.ncbi.nlm.nih.gov/35215639
초록/요약
In the growing polymer industry, the interest of researchers is captivated by bioplastics production with biodegradable and biocompatible properties. This study examines the polyhydroxyalkanoates (PHA) production performance of individual Lysinibacillus sp. RGS and Ralstonia eutropha ATCC 17699 and their co-culture by utilizing sugarcane bagasse (SCB) hydrolysates. Initially, acidic (H2SO4) and acidified sodium chlorite pretreatment was employed for the hydrolysis of SCB. The effects of chemical pretreatment on the SCB biomass assembly and its chemical constituents were studied by employing numerous analytical methods. Acidic pretreatment under optimal conditions showed effective delignification (60%) of the SCB biomass, leading to a maximum hydrolysis yield of 74.9 +/- 1.65% and a saccharification yield of 569.0 +/- 5.65 mg/g of SCB after enzymatic hydrolysis. The resulting SCB enzymatic hydrolysates were harnessed for PHA synthesis using individual microbial culture and their defined co-culture. Co-culture strategy was found to be effective in sugar assimilation, bacterial growth, and PHA production kinetic parameters relative to the individual strains. Furthermore, the effects of increasing acid pretreated SCB hydrolysates (20, 30, and 40 g/L) on cell density and PHA synthesis were studied. The effects of different cost-effective nutrient supplements and volatile fatty acids (VFAs) with acid pretreated SCB hydrolysates on cell growth and PHA production were studied. By employing optimal conditions and supplementation of corn steep liquor (CSL) and spent coffee waste extracted oil (SCGO), the co-culture produced maximum cell growth (DCW: 11.68 and 11.0 g/L), PHA accumulation (76% and 76%), and PHA titer (8.87 and 8.36 g/L), respectively. The findings collectively suggest that the development of a microbial co-culture strategy is a promising route for the efficient production of high-value bioplastics using different agricultural waste biomass.
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