Rhodamine-thiourea Linked Naphthalimide Derivative to Image ATP in Mitochondria using Two Channels
- 주제(키워드) fluorescent probe for ATP , mitochondria targeting , mitochondrial ATP , rhodamine
- 등재 SCIE, SCOPUS
- 발행기관 John Wiley and Sons Ltd
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000193622
- 본문언어 영어
- Published As https://doi.org/10.1002/asia.202200413
- PubMed https://pubmed.ncbi.nlm.nih.gov/35671139
초록/요약
Adenosine 5′-triphosphate (ATP), synthesized in mitochondria, is an energy molecule in all living things. ATP not only serves as an energy source for protein synthesis and muscle contraction, but also as an important indicator for various diseases, such as Parkinson's disease, cardiovascular disease, and others. Accordingly, detection and sensing of ATP, especially in mitochondria, are important. In this study, a unique ring-opening process of rhodamine was coupled to recognition of ATP via introduction of a thiourea moiety, which was further linked to a naphthalimide group. A strong fluorescent emission at ∼580 nm was accompanied by a color change from colorless to pink upon addition of ATP at pH 7.4. Fluorescent probe 1 successfully imaged mitochondrial ATP with a Pearson's coefficient of 0.8. In addition, green emission from the naphthalimide moiety at ∼530 nm was observed without any change upon addition of ATP. This emission can be considered equivalent to an internal standard to utilize probe 1 as a dual-channel probe for ATP. Furthermore, probe 1 showed negligible cytotoxicity based on MTT assays. © 2022 Wiley-VCH GmbH.
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