C-4-dicarboxylate metabolons: interaction of C-4-dicarboxylate transporters of Escherichia coli with cytosolic enzymes
- 주제(키워드) metabolon , C-4-dicarboxylate transporter , L-aspartate , Escherichia coli , BACTH , mSPINE
- 주제(기타) Microbiology
- 설명문(일반) [Schubert, Christopher; Unden, Gottfried] Johannes Gutenberg Univ Mainz, Inst Mol Physiol, Biozentrum 2, Hanns Dieter Husch Weg 17, D-55128 Mainz, Germany; [Kim, Nam Yeun; Bin Kim, Ok] Ewha Womans Univ, Dept Life Sci, Seoul 30760, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Submitted
- 발행기관 OXFORD UNIV PRESS
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000202968
- 본문언어 영어
- Published As https://doi.org/10.1093/femsle/fnac078
- PubMed https://pubmed.ncbi.nlm.nih.gov/36044995
초록/요약
Metabolons represent the structural organization of proteins for metabolic or regulatory pathways. Here, the interaction of fumarase FumB, aspartase AspA, and L-tartrate dehydratase TtdAB with the C4-dicarboxylate (C4-DC) transporters DcuA, DcuB, DcuC, and the L-tartrate transporter TtdT of Escherichia coli was tested by a bacterial two-hybrid (BACTH) assay in situ, or by co-chromatography using mSPINE (membrane Streptavidin protein interaction experiment). From the general C4-DC transporters, DcuB interacted with FumB and AspA, DcuA with AspA, whereas DcuC interacted with neither FumB nor AspA. Moreover, TtdT did not interact with TtdAB. The fumB-dcuB, the dcuA-aspA, and the ttdAB-ttdT genes encoding the respective proteins colocalize on the genome and each pair of genes forms cotranscripts, whereas the dcuC gene lies alone. The data suggest the formation of DcuB/FumB and DcuB/AspA metabolons for the uptake of L-malate, or L-aspartate, and their conversion to fumarate for fumarate respiration and excretion of the product succinate. The DcuA/AspA metabolon catalyzes uptake and conversion of L-aspartate to fumarate coupled to succinate excretion. The DcuA/AspA metabolon provides ammonia at the same time for nitrogen assimilation (ammonia shuttle). On the other hand, TtdT and TtdAB are not organized in a metabolon. Reasons for the formation (DcuA/AspA, DcuB/FumB, and DcuB/AspA) or nonformation (DcuC, TtdT, and TtdAB) of metabolons are discussed based on their metabolic roles. Experimental measurement for spatial proximity revealed that transporters for general C-4-dicarboxylates, such as L-malate and L-aspartate, form metabolons with cytosolic enzymes, however, no metabolons were observed for noncommon C-4-dicarboxylates.
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