Neutrophil Recruitment in Arterial Thrombus and Characteristics of Stroke Patients with Neutrophil-Rich Thrombus
- 주제(키워드) Neutrophil , thrombosis , stroke , neutrophil extracellular traps , intra-arterial thrombectomy
- 주제(기타) Medicine, General & Internal
- 설명문(일반) [Cha, Myoung-Jin; Lee, Hyungwoo; Kwon, Il; Kim, Sungeun; Kim, Young Dae; Nam, Hyo Suk; Heo, Ji Hoe] Yonsei Univ, Dept Neurol, Coll Med, Seoul, South Korea; [Cha, Myoung-Jin] Natl Police Hosp, Dept Neurol, Seoul, South Korea; [Ha, Jimin; Kwon, Il; Kim, Sungeun; Kim, Young Dae; Nam, Hyo Suk; Choi, Hyun-Jung; Heo, Ji Hoe] Yonsei Univ, Integrat Res Ctr Cerebrovasc & Cardiovasc Dis, Coll Med, Seoul, South Korea; [Lee, Hye Sun] Yonsei Univ, Coll Med, Biostat Collaborat Unit, Seoul, South Korea; [Song, Tae-Jin] Ewha Womans Univ, Dept Neurol, Sch Med, Seoul, South Korea; [Heo, Ji Hoe] Yonsei Univ, Dept Neurol, Coll Med, 50-1 Yonsei Ro, Seoul 03722, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published, gold
- 발행기관 YONSEI UNIV COLL MEDICINE
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000203040
- 본문언어 영어
- Published As https://doi.org/10.3349/ymj.2022.0328
- PubMed https://pubmed.ncbi.nlm.nih.gov/36303310
초록/요약
Purpose: Neutrophils contribute to thrombosis. However, there is limited information on the temporal course of neutrophil re-cruitment in thrombosis, the contribution of neutrophils to thrombus growth, and the characteristics of stroke patients with neu-trophil-rich thrombi.Materials and Methods: After inducing carotid artery thrombosis in Institute of Cancer Research mice using ferric chloride, aged thrombi were produced by ligating the distal portion of the carotid artery in mice for 0.5, 1, 2, 3, 6, or 24 h. For thrombus analysis in stroke patients, we used registry data and thrombi that were obtained during intra-arterial thrombectomy. Immunohisto-chemistry was performed to determine thrombus composition.Results: In the thrombi of 70 mice, Ly6G positive cell counts (neutrophils) and histone H3-positive cell counts increased in a time-dependent manner (both p<0.001). Ly6G-positive cell count was strongly correlated with histone H3-positive cell counts (r=0.910, p<0.001), but not with thrombus size (p=0.320). In 75 stroke patients, atrial fibrillation and cardioembolism were more fre-quent in the higher neutrophil group (32/37, 86.5%) than in the lower neutrophil group (19/38, 50%) (p=0.002). The median erythro-cyte fraction was higher [52.0 (interquartile range 39.9-57.8)] in the higher neutrophil group than in the lower neutrophil group [40.3 (interquartile range 23.5-53.2)]. The fraction of neutrophils was positively correlated with that of erythrocytes (R=0.35, p=0.002).Conclusion: Neutrophils were recruited and increased in arterial thrombosis in a time-dependent manner; however, they were not associated with the growth of formed thrombi. Neutrophil fractions in the thrombi of stroke patients appeared to be associat-ed with atrial fibrillation and erythrocyte fraction.
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