Biocompatible Properties and Mineralization Potential of Premixed Calcium Silicate-Based Cements and Fast-Set Calcium Silicate-Based Cements on Human Bone Marrow-Derived Mesenchymal Stem Cells
- 주제(키워드) calcium silicate-based cement , human bone marrow-derived mesenchymal stem cell , biocompatibility , mineralization potential
- 주제(기타) Chemistry, Physical; Materials Science, Multidisciplinary; Metallurgy & Metallurgical Engineering; Physics, Applied; Physics, Condensed Matter
- 설명문(일반) [Kim, Yemi] Ewha Womans Univ, Coll Med, Dept Conservat Dent, Seoul 07986, South Korea; [Lee, Donghee] Catholic Univ Korea, Coll Med, Dept Dent, Seoul 06591, South Korea; [Kye, Minjoo; Ha, Yun-Jae; Kim, Sin-Young] Catholic Univ Korea, Seoul St Marys Hosp, Coll Med, Dept Conservat Dent, Seoul 06591, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 MDPI
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000203094
- 본문언어 영어
- Published As https://doi.org/10.3390/ma15217595
- PubMed https://pubmed.ncbi.nlm.nih.gov/36363187
초록/요약
Premixed calcium silicate-based cements (CSCs) and fast-set CSCs were developed for the convenience of retrograde filling during endodontic microsurgery. The aim of this study was to analyze the biocompatible properties and mineralization potential of premixed CSCs, such as Endocem MTA Premixed (EM Premixed) and EndoSequence BC RRM putty (EndoSequence), and fast-set RetroMTA on human bone marrow-derived mesenchymal stem cells (BMSCs) compared to ProRoot MTA. Using CCK-8, a significantly higher proliferation of BMSCs occurred only in the EM Premixed group on days 2 and 4 (p < 0.05). On day 6, the ProRoot MTA group had significantly higher cell proliferation than the control group (p < 0.05). Regardless of the experimental materials, all groups had complete cell migration by day 4. Alizarin Red-S staining and alkaline phosphatase assay demonstrated higher mineralization potential of all CSCs similar to ProRoot MTA (p < 0.05). The EndoSequence group showed more upregulation of SMAD1 and OSX gene expression than the other experimental groups (p < 0.05), and all experimental cements upregulated osteogenic gene expression more than the control group (p < 0.05). Therefore, using premixed CSCs and fast-set CSCs as retrograde filling cements may facilitate satisfactory biological responses and comparable osteogenic potential to ProRoot MTA.
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