Label-free detection of leukemic myeloblasts in hyaluronic acid
- 주제(키워드) Microfluidic system , Motion microscopy , Myeloid leukemia , Vibration
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 BioMed Central Ltd
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000203190
- 본문언어 영어
- Published As https://doi.org/10.1186/s13036-022-00308-6
- PubMed https://pubmed.ncbi.nlm.nih.gov/36319989
초록/요약
Chronic myeloid leukemia is generally required bone marrow biopsy for diagnosis. Although examining peripheral blood is less invasive, it has not been fully validated as a routine diagnostic test due to suboptimal sensitivity. To overcome this limitation, a number of methodologies based on microfluidics have been developed for sorting circulating tumor cells from peripheral blood of patients with leukemia. In order to develop a more convenient method, we designed an analysis protocol using motion microscopy that amplifies cellular micro motions in a captured video by re-rendering pixels to generate extreme magnified visuals. Intriguingly, no fluctuations around leukemic myeloblasts were observed with a motion microscope at any wavelength of 0–10 Hz. However, use of 0.05% hyaluronic acid, one type of non-newtonian fluid, demonstrated fluctuations around leukemic myeloblasts under conditions of 25 μm/s and 0.5–1.5 Hz with a motion microscope. Thus, the non-invasive detection of leukemic myeloblasts can offer a valuable supplementary diagnostic tool for assessment of drug efficacy for monitoring patients with chronic myeloid leukemia. © 2022, The Author(s).
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