Synthesis of oligonucleotides containing 5′-homo-4′-selenouridine derivative and its increased resistance against nuclease
- 주제(키워드) 5′-Homo-4′-Selenonucleoside , Nuclease resistance , Oligonucleotide , Thermostability
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2023
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000203930
- 본문언어 영어
- Published As https://doi.org/10.1016/j.bmcl.2023.129172
초록/요약
As technologies using RNA or DNA have been developed, various chemical modifications of nucleosides have been attempted to increase the stability of oligonucleotides. Since it is known that 2′-OMe-modification greatly contributes to increasing the stability of oligonucleotides, we added 2′-OMe to our previously developed 4′-selenonucleoside and 5′-homo-4′-selenonucleoside as the modified monomers for oligonucleotide: 2′-methoxy-4′-selenouridine (2′-OMe-4′-Se-U) and 5′-homo-2′-methoxy-4′-selenouridine (5′-homo-2′-OMe-4′-Se-U). We synthesized oligonucleotides containing the chemically modified 4′-selenouridine and evaluated their thermal stability and nuclease resistance. In conclusion, the nuclease stability of the oligonucleotide containing 5′-homo-2′-OMe-4′-selenouridine increased while its thermal stability decreased. © 2023
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