Preparation of gamma-linolenic acid rich triacylglycerol from borage oil via a two-step lipase-catalyzed reaction
- 주제(키워드) borage oil , Candida rugosa lipase , enrichment , glyceride , triglyceride (TAG) , gamma-linolenic acid (GLA)
- 주제(기타) Chemistry, Applied; Food Science & Technology
- 설명문(일반) [Yoon, Hui Su; Lee, Yu Jin; Kim, In-Hwan] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, Transdisciplinary Major Learning Hlth Syst, Seoul, South Korea; [Kim, Byung Hee] Sookmyung Womens Univ, Dept Food & Nutr, Seoul, South Korea; [Kim, Yangha] Ewha Womans Univ, Dept Nutr Sci & Food Management, Seoul, South Korea; [Kim, In-Hwan] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, 145 Anam Ro, Seoul 02841, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 WILEY
- 발행년도 2023
- URI http://www.dcollection.net/handler/ewha/000000204223
- 본문언어 영어
- Published As https://doi.org/10.1002/aocs.12678
초록/요약
gamma-Linolenic acid (GLA) rich triacylglycerol (TAG) was successfully synthesized from glyceride, instead of glycerol, and fatty acid (FA) via Lipozyme TL IM-catalyzed esterification as a novel strategy. In the first step, GLA was enriched into glyceride fraction from borage oil by Candida rugosa lipase-catalyzed hydrolysis. The glyceride was separated from the reaction mixture by molecular distillation. GLA was enriched from 20.64% in borage oil to 45.94% in the glyceride fraction under optimum conditions. In the second step, the Lipozyme TL IM-catalyzed synthesis of TAG was carried out with the glyceride, and the FA obtained by saponification of a portion of the glyceride. The optimum conditions were the temperature of 50?, the enzyme loading of 10%, and the vacuum level of 20 mmHg, respectively. The maximum TAG content of approximately 92% was achieved after 12 h under the optimum conditions.
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