Comparative Analysis of Volatile and Non-Volatile Metabolites Derived from Bacillus subtilis Strains Producing Different Levels of Biogenic Amines
- 주제(키워드) biogenic amines , Bacillus subtilis , volatile metabolite , non-volatile metabolite
- 주제(기타) Biochemistry & Molecular Biology
- 설명문(일반) [Lee, Kyuwon; Kwon, Seo-Hee; Song, Sumin; Kim, Young-Suk] Ewha Womans Univ, Dept Food Sci & Biotechnol, Seoul 03760, South Korea; [Lee, Do-Yup] Seoul Natl Univ, Dept Food & Anim Biotechnol, Seoul 03760, South Korea; [Park, Min Kyung] Korea Food Res Inst, Food Proc Res Grp, Wonju 55365, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 MDPI
- 발행년도 2023
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000204247
- 본문언어 영어
- Published As https://doi.org/10.3390/metabo13020219
초록/요약
Biogenic amines (BAs), which are mainly generated by the microbial decarboxylation of amino acids, are important nitrogen compounds in fermented foods because of their toxicology. However, amino acids, the precursors of BAs, also play an important role in generating volatile and non-volatile metabolites, which are strongly associated with quality indicators for foods. Bacillus subtilis is one of dominant fermentative microorganism in various fermented foods and is well known as a BA-producing bacterium. In this study, B. subtilis strains which have different BAs-producing capacities, higher level of BAs production strain (BH) and lower level of BAs production strain (BL), were applied to compare the formations of volatile and non-volatile metabolite profiles according to cultivation times. In this study, histamine, putrescine, and spermidine were detected in all strains, however, 2-phenylethylamine was detected only in BH. Partial least squares discriminant analysis (PLS-DA) was applied to investigate the difference of metabolic profiles according to strains. In BH, some amino acids (phenylalanine, leucine, and threonine) and related volatile metabolites (3-methylbutanoic acid, pyrazines, styrene, and 1H-indole) were produced higher levels. On the other hand, BL produced significantly higher contents of metabolites associated with metabolism of fatty acids and nucleotides. It is necessary to consider the formation of metabolites in terms of quality as well as that of BAs during fermentation.
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