Cytoprotection of Probiotic Lactobacillus acidophilus with Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation
- 주제(키워드) coffee melanoidins , eggshell membrane hydrolysates , Lactobacillus acidophilus , single-cell nanoencapsulation , probiotics
- 주제(기타) Polymer Science
- 설명문(일반) [Han, Sang Yeong; Nguyen, Duc Tai; Kim, Nayoung; Kang, Eunhye K.; Choi, Insung S.] Korea Adv Inst Sci & Technol, Dept Chem, Ctr Cell Encapsulat Res, Daejeon 34141, South Korea; [Kim, Beom Jin] Univ Ulsan, Dept Chem, Ulsan 44776, South Korea; [Park, Ji Hun] Ewha Womans Univ, Dept Sci Educ, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 MDPI
- 발행년도 2023
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000204290
- 본문언어 영어
- Published As https://doi.org/10.3390/polym15051104
초록/요약
One-step fabrication method for thin films and shells is developed with nature-derived eggshell membrane hydrolysates (ESMHs) and coffee melanoidins (CMs) that have been discarded as food waste. The nature-derived polymeric materials, ESMHs and CMs, prove highly biocompatible with living cells, and the one-step method enables cytocompatible construction of cell-in-shell nanobiohybrid structures. Nanometric ESMH-CM shells are formed on individual probiotic Lactobacillus acidophilus, without any noticeable decrease in viability, and the ESMH-CM shells effectively protected L. acidophilus in the simulated gastric fluid (SGF). The cytoprotection power is further enhanced by Fe3+-mediated shell augmentation. For example, after 2 h of incubation in SGF, the viability of native L. acidophilus is 30%, whereas nanoencapsulated L. acidophilus, armed with the Fe3+-fortified ESMH-CM shells, show 79% in viability. The simple, time-efficient, and easy-to-process method developed in this work would contribute to many technological developments, including microbial biotherapeutics, as well as waste upcycling.
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