Adipose stem cell transplantation using adhesive protein-based viscous immiscible liquid for cartilage reconstruction
- 주제(키워드) Articular cartilage regeneration , Stem cell transplantation , Mussel adhesive protein , Complex coacervate
- 주제(기타) Engineering, Environmental; Engineering, Chemical
- 설명문(일반) [Maeng, Seong-Woo; Park, Tae Yoon; Yun, Jinyoung; Cha, Hyung Joon] Pohang Univ Sci & Technol, Dept Chem Engn, Pohang 37673, South Korea; [Ko, Ji-Yun; Park, So Hyun; Han, Sang Jun; Im, Gun-Il] Dongguk Univ, Res Inst Convergence Life Sci, Goyang 10326, South Korea; [Ko, Ji-Yun; Im, Gun-Il] Donnguk Univ, Dept Orthopaed, Ilsan Hosp Goyang, Seoul 10326, South Korea; [Joo, Kye Il] Ewha Womans Univ, Div Chem Engn & Mat Sci, Seoul 03760, South Korea; [Ha, Seongmin; Jee, Mingoo] Nat Gluetech Co Ltd, Seoul 08502, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCIENCE SA
- 발행년도 2023
- URI http://www.dcollection.net/handler/ewha/000000205915
- 본문언어 영어
- Published As https://doi.org/10.1016/j.cej.2023.142379
초록/요약
Articular cartilage (AC) is the smooth tissue that covers the end of bones and absorbs external impacts. AC can be damaged by various types of impacts. Thus, damaged AC can trigger the development of osteoarthritis (OA). With the limited intrinsic healing capacity of AC, cell transplantation has been recognized as a possible means of AC reconstitution. However, transplanted stem cells disappear from the transplantation site rapidly, abrogating the original intent of cell transplantation. In the present work, a viscous immiscible liquid-phase bioadhesive using bioengineered mussel adhesion protein (MAP) was applied for prolonged retention of transplanted stem cells in defective AC and retained stem cells in situ. Through electrostatic interactions with high-molecular-weight hyaluronic acid (HA), MAP was formulated into a viscous immiscible liquid phase complex coacervate (MAP-HA) with self-encapsulation of stem cells. MAP-HA exhibited excellent rheological and adhesive properties without cytotoxicity. Through in vivo rabbit OA model evaluation, we proved that immobilizing transplanted cells and preventing dispersion into the joint cavity by MAP-HA can increase the survival of transplanted cells and enhance their therapeutic effects. Therefore, stem cell-encapsulated biocompatible MAP-HA can be used as a cell-retaining bioadhesive scaffold in effective cartilage regeneration.
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