β-Ketoadipic acid production from poly(ethylene terephthalate) waste via chemobiological upcycling
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 Royal Society of Chemistry
- 발행년도 2023
- URI http://www.dcollection.net/handler/ewha/000000208782
- 본문언어 영어
- Published As https://doi.org/10.1039/d3ra02072j
- PubMed 37180017
초록/요약
The upcycling of poly(ethylene terephthalate) (PET) waste can simultaneously produce value-added chemicals and reduce the growing environmental impact of plastic waste. In this study, we designed a chemobiological system to convert terephthalic acid (TPA), an aromatic monomer of PET, to β-ketoadipic acid (βKA), a C6 keto-diacid that functions as a building block for nylon-6,6 analogs. Using microwave-assisted hydrolysis in a neutral aqueous system, PET was converted to TPA with Amberlyst-15, a conventional catalyst with high conversion efficiency and reusability. The bioconversion process of TPA into βKA used a recombinant Escherichia coli βKA expressing two conversion modules for TPA degradation (tphAabc and tphB) and βKA synthesis (aroY, catABC, and pcaD). To improve bioconversion, the formation of acetic acid, a deleterious factor for TPA conversion in flask cultivation, was efficiently regulated by deleting the poxB gene along with operating the bioreactor to supply oxygen. By applying two-stage fermentation consisting of the growth phase in pH 7 followed by the production phase in pH 5.5, a total of 13.61 mM βKA was successfully produced with 96% conversion efficiency. This efficient chemobiological PET upcycling system provides a promising approach for the circular economy to acquire various chemicals from PET waste. © 2023 The Royal Society of Chemistry.
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