Eye-Mimicked Neural Network Composed of Photosensitive Neural Spheroids with Human Opsin Proteins
- 주제(키워드) Brain , Eye protection , Light sensitive materials , Neural networks , Proteins , Biological mimicing , Human opsin protein , In-vitro models , Neural-networks , Photosensitive cell , Purple lights , Reaction under , Selective reactions , Short wavelengths , Spheroid , Photosensitivity
- 등재 SCIE, SCOPUS
- 발행기관 John Wiley and Sons Inc
- 발행년도 2023
- URI http://www.dcollection.net/handler/ewha/000000209412
- 본문언어 영어
- Published As https://doi.org/10.1002/adma.202302996
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
An in vitro model, composed of the short-wavelength human opsins and rhodopsins, is created. Two types of photosensitive neural spheroids are transfected for selective reaction under bluish-purple and green lights. These are employed to two devices with intact neuron and neural-spheroid to study the interaction. By photostimulation, the photosensitive spheroid initiated photoactivation, and the signal generated from its body is transmitted to adjacent neural networks. Specifically, the signal traveled through the axon bundle in narrow gap from photosensitive spheroid to intact spheroid as an eye-to-brain model including optic nerve. The whole process with photosensitive spheroid is monitored by calcium ion detecting fluorescence images. The results of this study can be applied to examine vision restoration and novel photosensitive biological systems with spectral sensitivity. © 2023 Wiley-VCH GmbH.
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