A tryptophan-based assay method to search regulatory compounds for transcriptionally controlled tumor protein
- 주제(키워드) Flavonoid , Fluorescence , Native gel electrophoresis , Transcriptionally controlled tumor protein (TCTP) , Tryptophan
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier B.V.
- 발행년도 2024
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000213484
- 본문언어 영어
- Published As https://doi.org/10.1016/j.bbrc.2023.149363
- PubMed 38071892
초록/요약
Transcriptionally controlled tumor protein (TCTP) is a highly conserved protein performing a large number of cellular functions by binding with various partner proteins. The importance of its roles in many diseases requires an assay method to find regulatory compounds. However, the molecular characteristics of TCTP made it difficult to search for chemicals interacting with it. In this study, a tryptophan-based assay method was designed and Y151W mutant TCTP was constructed to search binding chemicals. Since there is no tryptophan in the native sequence of TCTP, the incorporation of tryptophan in the Y151W mutant was very effective to establish the method. A flavonoid library was employed to the assay with the method. With the native and Y151W mutant TCTPs, three flavonoids such as morin, myricetin and isobavachalcone have been found to interact with TCTP. Combined with native gel electrophoresis, the binding region of isobavachalcone was suggested to be the flexible loop of TCTP. This approach can be easily applicable to find binding compounds of proteins with similar molecular characteristics of TCTP. © 2023
more