Bioadhesive levan-based coaxial nanofibrous membranes with enhanced cell adhesion and mesenchymal stem cell differentiation
- 주제(키워드) Composite nanofibrous membrane , Levan , Coaxial electrospinning , Cell-based tissue engineering
- 주제(기타) Chemistry, Applied; Chemistry, Organic; Polymer Science
- 설명문(일반) [Ji, Eunhyun; Kim, Yesol; Lee, Eunji; Joo, Kye Il] Ewha Womans Univ, Dept Chem Engn & Mat Sci, Seoul 03760, South Korea; [Song, Young Hoon; Lee, Jae Kyeong; Seo, Jeong Hyun] Yeungnam Univ, Sch Chem Engn, 280 Daehakro, Gyongsan 38541, Gyeongbuk, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCI LTD
- 발행년도 2025
- URI http://www.dcollection.net/handler/ewha/000000245586
- 본문언어 영어
- Published As https://doi.org/10.1016/j.carbpol.2025.123337
- PubMed https://pubmed.ncbi.nlm.nih.gov/39978912
초록/요약
Conventional electrospun nanofibrous membranes have been widely used for tissue engineering scaffolds because they can mimic extracellular matrix (ECM), which plays a significant role in cell proliferation, adhesion, and differentiation. However, the inadequate mechanical strength and biological functions of electrospun nanofibrous scaffolds limit the range of their practical applications. In this study, we prepared a uniform levanbased core-shell composite (csCAL) nanofibrous membrane using the coaxial electrospinning technique. The coaxial csCAL membrane with levan and cellulose acetate (CA) as shell and core, respectively, exhibited highly enhanced mechanical properties and adhesive strength. Moreover, the unique bioadhesive nature of these membranes significantly enhanced cell attachment and proliferation, while their high biocompatibility and biodegradability hold substantial promise for application as functional cell carriers. Upon incorporating mesenchymal stem cells (MSCs) into the csCAL nanofibrous membrane, we observed enhanced osteogenesis and chondrogenesis, as evidenced by alizarin red and alcian blue staining, respectively. These results indicate that the levan-based nanofiber architecture has the potential to deliver scaffolds for supporting the differentiation of MSCs.
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