Synthesis of pinolenic acid-enriched triacylglycerol from pine nut oil via a two-step consecutive enzyme reaction: Comparison of acyl donors
- 주제(키워드) acyl donors , Candida antarctica , enrichment , Lipozyme 435 , pinolenic acid , triglycerides
- 주제(기타) Chemistry, Applied; Food Science & Technology
- 설명문(일반) [Lee, Yu Jin; Park, Mi Soon; Kim, In-Hwan] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, Transdisciplinary Major Learning Hlth Syst, Seoul, South Korea; [Kim, Byung Hee] Sookmyung Womens Univ, Dept Food & Nutr, Seoul, South Korea; [Kim, Yangha] Ewha Womans Univ, Dept Nutr Sci & Food Management, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 WILEY
- 발행년도 2025
- URI http://www.dcollection.net/handler/ewha/000000245607
- 본문언어 영어
- Published As https://doi.org/10.1002/aocs.12881
초록/요약
Pinolenic acid (PLA) is a plant-origin Delta 5-unsaturated polymethylene-interrupted fatty acid that provides several beneficial health effects to the human body. A two-step lipase-catalyzed reaction was carried out to synthesize PLA-enriched triacylglycerol (TAG) with pine nut oil (PNO) in the present study. In the first step, PLA was efficiently enriched from an initial value of 15-43 mol% in ethyl ester of the reaction mixture after Lipozyme 435-catalyzed ethanolysis with PNO. In the second step, PLA-enriched TAG was efficiently synthesized with the fatty acid form via Lipozyme 435-catalyzed esterification at all temperatures when both ethyl ester form and fatty acid form were compared as acyl donors. The effect of vacuum on the synthesis of PLA-enriched TAG with glycerol and PLA-enriched fatty acid was studied. The optimum temperature and vacuum were 60 degrees C, and 50 mmHg, respectively. A maximum TAG conversion of approximately 95% was achieved after 12 h under the optimum conditions.
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