포도당에 따른 이자섬의 분비단백질체 분석
Glucose Condition-Dependent Pancreatic Islet Secretomic Profiling
- 주제(키워드) pancreatic islet , secretomics , differential analysis
- 발행기관 포항공과대학교 일반대학원
- 지도교수 류성호
- 발행년도 2013
- 학위수여년월 2013. 2
- 학위명 석사
- 학과 및 전공 일반대학원 융합생명공학부
- 세부전공 신호전달 및 단백질체학
- 원문페이지 49
- 실제URI http://www.dcollection.net/handler/postech/000001557026
- 본문언어 영어
- 저작권 포항공과대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
As an endocrine organ, the pancreatic islet secretes several hormones including insulin and glucagon. However, to date no studies have focused on the pancreatic islet secretome. Therefore, it is necessary to profile the pancreatic islet secretome, which may be dependent on the glucose condition, and to identify changes in the secreted protein pool following various glucose concentration stimuli. In this study, primary islets were prepared from C57BL/6 adult mice and treated with various glucose concentrations. A comparative secretomic analysis was then conducted. Ions and glucose were removed by a centrifugal filter in conditioned media and fractionated. The exosomes were then analyzed for peptides by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS). Proteins such as MIF, ENO2, and GSTM1 were identified after filtering. We found an increase in ENO2 and MIF and a decrease in GSTM1 in the high glucose condition compared to the low glucose condition. This was the first systematic unbiased study of the pancreatic islet secretome, and the results suggest that novel secretory factors may be dependent on glucose levels.
more목차
Abstract ------------------------------------ I
Contents ----------------------------------- III
Introduction --------------------------------- 1
Materials and Methods --------------------- 3
Results ------------------------------------ 7
Discussion --------------------------------- 11
국문요약 ----------------------------------- 35
References -------------------------------- 36
Acknowledgements ------------------------ 40