대 체적 골조직재생을 위한 치수줄기세포와 두 가지 성장인자가 프린팅 된 구조체의 개발
Development of DPSC/dual growth factor printed structure for vascularized large volume bone regeneration
- 발행기관 포항공과대학교 일반대학원
- 지도교수 조동우
- 발행년도 2013
- 학위수여년월 2013. 8
- 학위명 석사
- 학과 및 전공 일반대학원 융합생명공학부
- 실제URI http://www.dcollection.net/handler/postech/000001628307
- 본문언어 한국어
- 저작권 포항공과대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Vascularized tissue regeneration is one of main issues for sufficient nutrition transport. When the engineered structure is implanted, tissue regeneration depends on surrounding diffusive nutrient supply and waste removal processes until the target tissue becomes vascularized. The aim of this study was to build a three-dimensional structure with vascular networks for bone tissue regeneration. To offer a vascularized condition, rapid prototype-based structure was designed to control the position and release time of dual growth factors. Cell source was dental pulp stem cell (DPSC) which has multipotency into both vascular networks and bone by dual growth factors; VEGF and BMP-2. Two types of hydrogel, which were able to control the release time of dual growth factors, were dispensed at the desired position with cell. Finally, various assays such as histochemical/immunohistochemical analyses, ELISA, and real time PCR were carried out to prove the effect of designed structure in both in vitro and in vivo.
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