허리 디스크 탈출증이 있는 통증 쥐의 척수에서 등쪽 뿔안의 AMPA 수용체의 변화에 대한 연구
Changes of AMPA receptors within Dorsal horn of spinal cord in pain rat model of lumbar disc herniation
- 주제(키워드) AMPA receptor , Intervertebral disc displacement , spinal cord dorsal horn
- 발행기관 영남대학교 대학원
- 지도교수 이동규
- 발행년도 2020
- 학위수여년월 2020. 2
- 학위명 박사
- 학과 및 전공 대학원 의학과
- 세부전공 재활의학
- 실제URI http://www.dcollection.net/handler/yu/200000282581
- UCI I804:47017-200000282581
- 본문언어 영어
- 저작권 영남대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Background: α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors are involved in central sensitization after inflammation in the spinal dorsal horn. Glial cells have a key role in maintaining central sensitization. Therefore, we investigate the glial cell and AMPA receptor activity over time after surgery for disc herniation. Methods: In total, 70 Sprague-Dawley rats were randomly assigned to the following groups: Control (n = 6), sham-operated (n = 4), and nucleus pulposus-exposed groups for AMPA receptors (n = 30) and glial cell (n = 40). The rats were tested for mechanical allodynia; immunohistochemical staining for AMPA receptors (GluA1 and GluA2) and glial cells (OX-42 and glial fibrillary acid protein [GFAP]) in the spinal dorsal horn was performed on postoperative days 3, 7, and 14. Results: Mechanical withdrawal thresholds decreased after surgery, and this effect was maintained for up to 14 days. Immunohistochemical expression of GluA1 and GluA2 in the spinal dorsal horn increased quantitatively on postoperative days 3 and 7 (P < 0.05) and reached levels similar to that of the control on postoperative day 14. Moreover, immunohistochemical expression of OX-42 and GFAP showed similar changes in AMPA receptors after surgery. Although the activity of AMPA receptors and glial cells achieved normalcy, mechanical withdrawal threshold of the hind paw remained decreased even 38 days after surgery. Conclusions: The rat model of lumbar disc herniation showed increased expression of AMPA receptor and glial cell activity in the spinal dorsal horn. Neuropathic pain following disc herniation can be managed without active inflammation.
more목차
Introduction 1
Methods 3
Results 7
Discussion 15
Conclusion 22
References 23
Korean abstract 26